THE PITCHER LIQUOR OF NEPENTHES. 



117 



experiment, save in tliose indicated by an asterisk (*) in the tabu- 

 lated results. In the latter experiments, composite samples of 

 liquor, obtained from several pitchers, were used. 



In the preliminary experiments, the carmine fibrin was swollen 

 in a solution containing 0.2 per cent, hydrochloric acid and 0.2 per 

 cent, trikresol. A definite volume of the resulting jelly was placed 

 in a given volume of pitcher liquor ; and sufficient hydrochloric acid 

 (0.6 per cent, solution) and trikresol (2 per cent, solution) were 

 added to produce a concentration of 0.2 per cent, of each of these 

 reagents. The time required for solution of the substrate and 

 other details of each experiment are given in Table III. 



TABLE IIL 



Digestion of Gelatinous Carmine Fibrin by Pitcher Liquor in the 

 Presence of 0.2 Per Cent. Hydrochloric Acid. 



In the final series of experiments, 0.2 gram of carmine fibrin 

 was weighed out into a separate tube for each experiment. This 

 series falls into three groups. In group A, the carmine fibrin was 

 swollen in its tube in a solution containing 0.2 per cent, hydrochloric 

 acid and 0.2 per cent, trikresol, then was transferred to another 

 tube and immediately used in a digestion experiment. In groups 

 B and C, unswollen carmine fibrin was used. No hydrochloric acid 

 was used in group B ; in the other two groups, sufficient 0.6 per 

 cent, hydrochloric acid was added to render the final concentration 

 of that acid 0.2 per cent. Trikresol was used as a bactericide in all 

 three groups, adding sufficient of its 2 per cent, aqueous solution to 

 produce a final concentration of 0.2 per cent. The details of these 

 experiments are recorded in Table IV. 



PROC. AMER. PHIL. SOC, VOL. LVII, I, JUNE I4, I918. 



