28 



C. L. SADRON 



If, by chance, the measured value of M w has a close connection to the 

 actual mean molecular weight of the particles, the conclusion would be 

 that the group II particles are dimers of particles of group I. 



5. At any rate, with particles treated with chymotrypsin, the compli- 

 cated effects that we have mentioned in point 2, disappear completely. For 

 instance, curve III in Fig. 10 shows that the value of M w for sample AMJi 

 after treatment is quite independent of the concentration of NaCl and 

 remains at 7 X 10 , a value characteristic of group I. 



For this reason, we shall, in the following, devote our principal attention 

 to samples which were preliminarily treated with the enzyme. 



c. Light Scattering at Large Angles 



Since the values of hiL seem to be so large that the admissibility of the 

 extrapolation to zero values of 6 must be questioned, it is quite logical to 

 investigate if — on the contrary — we can use the experimental data to as- 

 certain the asymptotic values of c/K at the other end, that is when hL 

 goes to infinity. 



For this purpose we shall plot the values of c/K as a function of sin 6/2, 



Sin 9/2 



0.2 0.3 4 0.5 0.6 0.7 0.8 0.9 

 Sin 9/2 



(A) (B) 



Fig. 12. A: c/K as a function of sin 6/2 for MAV (not treated). The three curves 

 are respectively relative to solutions of 1, 0.5, and 0.1 molar NaCl (downward). B: 

 c/K as a function of sin 9/2 for MAV (treated). The effect of the concentration of 

 NaCl has practically disappeared; # = saturated NaCl, X = 1 molar NaCl, O = 

 0.25 molar NaCl. 



