BIOLOGY OF SPERMATOZOA 



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MINUTES 

 Fig. 13.5. Heart rate of man during coitus recorded by cardiotachometer. (From E. P. 

 Boas and E. F. Goldschmidt, The Heart Rate, Charles C Thomas, 1932.) 



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tion between cerebral and spinal centers 

 warrants considerable further study. The 

 subject is further discussed in the chapter 

 by ]\Ioney. Ejaculation in men and dogs is 

 accompanied by pronounced cardiovascular 

 intensification (Pussep, 1921; Boas and 

 (ioldschmidt, 1932; Bartlett, 1956) affecting 

 both blood pressure and pulse rate (Figs. 

 13.5, 13.6). Androgen administration in- 

 creases libido and copulatory arousal (see 

 cha])ter by Young ) , but the manner in which 

 this is related to the preceding neurogenic 

 factors is not well understood (Cheng and 

 Casida. 1949). 



B. COLLECTION OF SEMINAL COMPONENTS 



Various methods of seminal collection 

 may be employed for cither whole or frac- 

 tional analyses. Normal ejaculates are 

 cxix'ctcd when induced l)y masturbation, 

 electrical stinuilation, or discharge into an 

 artificial vagina. Sperm samples without 



seminal plasma are readily obtainable from 

 the epididymis and vas deferens of the ex- 

 cised tracts of many animals (e.g., the 

 guinea pig, rat, boar, bull, and stallion) by 

 backflushing the vas and cutting the epi- 

 didymis. Relatively uncontaminated pros- 

 tatic fluid is procurable, e.g., from men and 

 dogs, and vesicular fluid from men, by man- 

 ual massage of the appropriate glandular 

 regions. Incomjilete ejaculates are produced 

 after extirpation of such organs as the semi- 

 nal vesicles and Cowper's glands; the pros- 

 tatic isolation operation, perfected on dogs 

 l)y Huggins, Masina, Eichelberger and 

 Wharton (1939) and illustrated in Chapter 

 6. Figure 6.2, permits the collection of large 

 amounts of uncontaminated prostatic fluid. 



C. SEMINAL VOLUME AND SUCCESSIVE 

 FRACTIONS 



Scniiiial xolunie bears some relation to 

 animal sizt'. but the individual contril)utions 



