300 



2. ANALOGS OF ENZYME REACTION COMPONENTS 



lustrates the importance of taking ionization into account in the comparison 

 of inhibitors, as discussed earlier in the chapter. It must be emphasized 

 that the absolute values of the binding energy are meaningless; it is only 

 the differences between the — JF values that are significant. 



Before discussing the implications of these results we will examine the 

 data reported by Krueger (1955) on the inhibition of a mushroom enzyme 

 oxidizing p-cresol (Table 2-8). Confirming the work of Kuttner and Wag- 



Table 2-8 

 Inhibition of p-Cresol Oxidation by Mushroom Tyrosinase ' 



" Concentration of p-cresol was 4.63 mM except where indicated by asterisks, in 

 which cases it was 9.26 n\M. The relative binding energies were calculated for pH 5.3 

 and were not corrected for ionization. (Data from Krueger, 1955.) 



