392 



2. ANALOGS OF ENZYME REACTION COMPONENTS 



100 



50 



% 



INH 



ANAEROBIC 

 GLYCOLYSIS 



0.1 



(2-06) 



Fig. 2-10. Effects of 2-DG on the glucose metabolism in cat brain slices. 

 (From Tower, 1958.) 



respiration. The small effect on galactose respiration was felt to be due 

 perhaps to a different hexokinase being used for the phosphorylation of 

 galactose, since inhibition is exerted by 2- and 4-deoxy galactose. 



Glycolysis as measured by the formation of C^^Og from glucose-u-C^^ 

 is depressed by 2-DG in diaphragm (Nakada and Wick, 1956), kidney (Serif 

 and Wick, 1958), lymph node cells (Helmreich and Eisen, 1959), and adipose 

 tissue (Brooks et at., 1961). The variation of inhibition with 2-DG concen- 

 tration is shown in Fig. 2-11 for rat kidney slices. Glycolysis as measured 

 by unlabeled COg or lactate formation is also inhibited in yeast (Cramer 

 and Woodward, 1952), ascites carcinoma and leukemic cells (Laszlo et al., 

 1958), and cerebral cortex slices (Tower, 1958). The depression of aerobic, 

 and anaerobic glycolysis in tumor tissue is counteracted by increasing glu- 

 cose concentrations (Woodward and Hudson, 1954). These results are quite 



