ANALOGS OF NICOTINAMIDE 



501 



Inhibitor 



Relative "KT " 



Lactate DH 



Glucose DH 



Salicylate 



p-Aminobenzoate 



Salicylamide 



Pyridine-3-sulfonate 



Nicotinate 



Benzenesulfonate 



m - Aminobenzoate 



Benzoate 



Pyridine-3-sulfonamide 



Nicotinamide 



Benzamide 



Trigonelline 



Adenosine 



Adenosine-3'-P 



ATP 



" The relative "K/' values were calculated from the inhibitions at varying con- 

 centrations in order that the relative potencies of the inhibitors could be more readily 

 compared; they are not absolute values. 



Stockholm but determined the inhibitor constants by plotting, so that the 

 values in the accompanying tabulation for beef liver glucose dehydrogenase 



Inhibitor 



Ki Relative — ZiF of binding 



(mM) (kcal/mole) 



4-Pyridoxate 



Pyridoxal 



3-Hydroxypyridine 



Nicotinate 



2-Methylnicotinat« 



Nicotinamide 



Isonicotinyl hydrazide 



Pyridine 



Isonicotinate 



Trigonelline 



ATP 



Adenosine-3'-P 



Adenosine 



Adenine 



Phosphate 



