INHIBITION OF ENZYMES 



803 



8- 



0.6 



0.2- 



FiG. 7-20. Theoretical curves showing the relationship between 

 reaction of enzyme SH groups and inhibition of the enzyme ac- 

 tivity for an enzyme containing two SH groups, only one of which 

 is involved in the catalytic activity. (SHi)^ = (SHa)^ = 0.01 mM 

 and K2/K1 varied as indicated by the numbers on the curves; r is 

 the fraction of SH groups reacted and is represented by the 

 straight line. It is assumed that free mercurial is zero. 



Type B 



Catalase — beef liver (Schiitte and Niirnberger, 1959) 

 Enolase — rabbit muscle (Holt and Wold, 1961) 

 Hexokinase — yeast (Fasella and Hammes, 1963) 



Type C 



ATPase — moysin (Singer and Barron, 1944; Barany, 1959; Gilmour and Gellert, 



1961) 

 Alcohol dehydrogenase — yeast (Barron and Levine, 1952) 



Aldolase — rabbit muscle (Swenson and Boyer, 1957; Szabolcsi and Biszku, 1961) 

 /?- Amylase — barley (Rowe and Weill, 1962) 

 Malate dehydrogenase — pig heart (Thome and Kaplan, 1963) 

 Phosphorylase — rabbit muscle (Madsen and Cori, 1955, 1956) 

 Rhodanese — beef liver (Sorbo, 1963) 

 Urease — jack bean (Hellerman et ah, 1943) 

 Xanthine oxidase — milk (Gilbert, 1963) 



Type D 



Aldehyde oxidase — rabbit liver (Rajagopalan et al., 1962) 



