AMOEBINA 459 



trodon mokasen, Natrix rhombifer, N. sipedon, N. sipedon sipedon, 

 N. cyclopion, Python sebae, Rachidelus brazili, etc. Zoological Gar- 

 dens in Philadelphia (Geiman and Ratcliffe) and Antwerp (Rodhain). 



The amoeba produces lesions in the stomach, duodenum, ileum, 

 colon and liver in host animals. Time for excystation in host's intes- 

 tine (jejunum and ileum) five to 14 hours; time for metacystic devel- 

 opment in host's intestine seven-24 hours; the excysted amoeba with 

 four nuclei, each of which divides once, divides finally into eight 

 amoebulae; optimum temperature for culture 20-30°C. (Geiman and 

 Ratcliffe, 1936). Ratcliffe and Geiman (1938) observed spontaneous 

 and experimental amoebiasis in 32 reptiles. 



E. ranarum (Grassi). In colon of various species of frogs; re- 

 sembles E. histolytica; 10-50/x in diameter; cysts are usually tetranu- 

 cleate, but some contain as many as 16 nuclei; amoebic abscess of 

 the liver was reported in one frog. Comparison with E. histolytica 

 (Dobell, 1918); life cycle (Sanders, 1931). 



E. (?) phallusiae Mackinnon and Ray. In the intestine of the ascid- 

 ian, Phallusia mamillata; 15-30m by 10-15m; nucleus about 5ju in 

 diameter, structure not well defined; cysts uninucleate, about 20/z 

 in diameter; parasitic nutrition. 



E. minchini Mackinnon. In gut of tipulid larvae; 5-30/x in diam- 

 eter; cyst nuclei up to 10 in number. 



E. apis Fantham and Porter. In Apis mellifica; similar to E. coli. 



E. thomsoni Lucas. In the colon of cockroaches; when rounded 

 7-30 (15-25)m in diameter; usually attached to debris by a knob- 

 like process, highly adhesive; cytoplasm poorly differentiated; vesic- 

 ular nucleus with peripheral granules; endosome variable, with 

 loosely aggregated granules and a central dot; cysts 8-16/x in diame- 

 ter, with one to four nuclei (Lucas, 1927). 



E. aulastomi Noller. In the gut of the horse-leech, Haemopis san- 

 guisuga; cysts with four nuclei. Morphology nad development 

 (Bishop, 1932). 



E. paulista (Carini) (Brumptina paulista C.) (Fig. 189, c). In the 

 cytoplasm of many species of Protociliata; trophozoites 5.3-14. 3/z 

 in diameter; cysts about 9.4/x in diameter, uninucleate; no effect upon 

 host ciliates even in case of heavy infection (Stabler and Chen, 1936; 

 Chen and Stabler, 1936). Carini and Reichenow (1935) : trophozoites 

 8-14jii in diameter; cysts 8-12/i; either identical with E. ranarum or 

 a race derived from it. 



Genus Iodamoeba Dobell. Vesicular nucleus, with a large en- 

 dosome rich in chromatin, a layer of globules which surrounds the 

 endosome and do not stain deeply, and achromatic strands between 



