882 PROTOZOOLOGY 



factory one is as follows: Fill a finger bowl with about 150 cc. of glass 

 distilled water and place 4 rice grains on the bottom. Let the dish 

 stand for a few days, and then introduce with a pipette a number of 

 desired flagellates from a mass culture into it. Cover the bowl and 

 keep it at about 20° C. 



Mast (1939) used the following media for Chilomonas Paramecium. 



(a) Glucose-peptone solution: 



Peptone 8 gm. 



Glucose 2 gm. 



Water 1000 cc. 



Amoeba proteus and other freshwater amoebae. — Fill a finger bowl 

 with 200 cc. of glass distilled water, and place 4 rice grains. After a 

 few days seed with amoebae (p. 879), add about 5 cc. of Chilomonas 

 culture, and cover the bowl with a glass cover. In about two weeks 

 a ring of amoebae will be found around each rice grain, and if Chilo- 

 monas do not overmultiply, the amoebae will be found abundantly in 

 another two weeks. If properly maintained, subcultures may be 

 made every 4-6 weeks. Chalkley (1930) advocates substitution of 

 the plain water with a salt solution which is composed of 



NaCl 0.1 gm. 



KC1 0.004 gm. 



CaCl 2 0.006 gm. 



Glass distilled water 1000 cc. 



If the culture water becomes turbid, make subcultures or pour off 

 the water and fill with fresh distilled water or the solution. Culture 

 should be kept at 18-22°C. 



Hahnert (1932) used the following culture solution: 



KC1 0.004 gm. 



CaCl 2 0.004 gm. 



CaH 4 (P0 4 ) 2 0.002 gm. 



Mg 3 (P0 4 ) 2 0.002 gm. 



