884 



PROTOZOOLOGY 



as submerged leaves or surface scum containing the ciliates. If de- 

 sired, culture may be started with a single individual in a watch 

 glass. Collection, cultivation and sterilization of Paramecium (Wich- 

 terman, 1949). 



Pure culture 



Many free-living flagellates and certain ciliates have in recent 

 years been successfully cultured free from any other associated or- 

 ganisms. The protozoan to be cultivated must be freed from other 

 Protozoa and bacteria. For this, washing, dilution, migration and 

 bactericidal agents have been used. For information, the reader is 

 referred to Glaser and Coria (1930), Claff (1940), Taylor and Van 

 Wagentock (1941), Kidder (1941), etc. 



Free-living Phytomastigina. — Many media are known. See Prings- 

 heim (1926, 1937, 1946), Hall (1937, 1941), Hutner and Provasoli 

 (1951), etc. 



Tetrahymena and allied forms. — Kidder, Dewey and Parks use a 

 basal medium as quoted below: 

 y per ml 



DL-alanine 110 



L-arginine 206 



L-aspartic acid 122 



Glycine 10 



L-glutamic acid 233 



L-histidine 87 



DL-isoleucine 276 



L-leucine 344 



L-lysine 272 



DL-methionine 248 



L-phenylalanine 160 



L-proline 250 



DL-serine 394 



DL-threonine 326 



L-tryptophane 72 



DL-valine 162 



7 per ml 



Thiamine HC1 1.00 



Biotin (free acid) 0.0005 



Choline CI 1.00 



MgSo 4 -7H 2 0. . 

 Fe(NH 4 ) 2 (S0 4 ) 2 

 MnCl 2 -4H 2 0. . 



ZnCl 2 



CaCl 2 -2H 2 0... 

 CuCl 2 -2H 2 0... 

 FeCL-6H 2 0. .. 



K 2 HP0 4 



KH 2 OP 4 



6H 2 



Ca pantothenate 0.10 



Nicotinamide 0.10 



Pyridoxine HC1 1.00 



Pyridoxal HC1 0.10 



Pyridoxamine HC1 0. 10 



Riboflavin 0.10 



Pteroylglutamic acid 0.01 



Guanylic acid . 

 Adenylic acid. 

 Cytidylic acid . 

 Uracil 



100 



25 

 0.5 

 0.05 



50 



5 



1.25 



1,000 



1,000 



30 

 20 

 25 

 10 



Dextrose 2,500 



Na acetate 1,000 



Tween85 700 



Protogen 1 unit 



B. Parasitic Protozoa 



Intestinal flagellates of man. — There are numerous media which 

 have been used successfully by several investigators. 



