COLLECTION, CULTIVATION, OBSERVATION 



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(a) Ovo-mucoid medium (Hogue, 1921). White of two eggs are 

 broken in a sterile flask with beads. Add 200 cc. of 0.7 % NaCl solu- 

 tion and cook the whole for 30 minutes over a boiling water bath, 

 shaking the mixture constantly. Filter through a coarse cheese cloth 

 and through cotton-wool with the aid of a suction pump. Put 6 cc. of 

 the filtrate in each test tube. Autoclave the tubes for 20 minutes un- 

 der 15 pounds pressure. After cooling, a small amount of fresh faecal 

 material containing the flagellates is introduced into the tubes. Incu- 

 bate at 37°C. 



(b) Sodium chloride sheep serum water (Hogue, 1922). Composed 

 of 100 cc. of sterile 0.95% NaCl and 10-15 cc. of sterile sheep serum 

 water (dilution 1:3). 15 cc. to each tube. Trichomonas hominis, T. 

 tenax, and Retortamonas intestinalis grow well. 



Trichomonas vaginalis. — Johnson and Trussell (1943) reported the 

 following mixture the most suitable medium : 



Heat the mixture in a water bath to melt the agar; filter through a 

 coarse paper; add 0.7 cc. of 0.5 per cent aqueous methylene blue; 

 adjust pH to 5.8-6.0 with N/1 HC1 or NaOH; tube 8 cc; autoclave. 

 After cooling, add aseptically 2 cc. of sterile (filtered) human serum. 

 Incubate at least four days; store at room temperature for two to 

 three weeks or as long as an amber "anaerobic" zone is apparent. 

 Termite flagellates. — Trager's (1934) media are as follows: 



