GENERAL METABOLISM ttl VlttO 



79 



Table 12. — Quantities of Acid-soluble Phosphates In Cerebral 



Cortical Slices in vitro after Incubation in Salines 



Containing Glucose 



Superscripts denote references below 



Generally, salines contained: NaCl 125 mM, KCl 6-3 mM, CaCla 2-7 

 niM, MgS04 1-28 mM, glucose 10-0 mM, and either sodium phosphate 

 pH 7-4 01 M, or 2-amino-2(hydroxymethyl)-l : 3-propanediol pH 7-4 

 50 mM. 



<i) Guinea pig, Heald, 1956«. (2) Guinea pig, Heald 1957. (3) Guinea 

 pig, Thomas, 1957. (*) Guinea pig, Heald, 1954. (5) Mcllwain et aL, 

 1951. (®) Guinea pig, Kratzing and Narayanaswami, 1953. C) Rat, 

 Heald, unpublished. (^) Human, leucotomy specimens, Heald, unpub- 

 lished. (^) Human, leucotomy specimen, Greengard (1955). d") Cat, 

 Tower, 1958. The medium contained 27 mM potassium salts. dD Guinea 

 pig. Gore et aL, 1950. 



and the surrounding medium is much more rapid. Thus on 

 incubation in saline containing glucose, both in the presence and 

 absence of inorganic phosphate, guinea pig cerebral slices lost 

 3-0-4-Oju-moles of phosphate/g wet wt. during a period of 30-90 

 min (Mcllwain et aL, 1951; Heald, \9S6b). Comparison of the 

 quantity lost with the amounts present as inorganic phosphate in the 

 tissue suggests that part of the phosphate appearing in the medium 



