GENERAL METABOLISM Itl Vltro 



83 



Table 13. — Oxidative Phosphorylation in Cerebral Mitochondria 



During the Metabolism of Different Substrates 



Superscripts denote references below 



<i) Brody and Bain (1952), period of incubation 30 min. ^^^ Abood and 

 Gerard (1952), period of incubation 20 min. ^^^ Abood and Romanchek 

 (1955), period of incubation 10 min. ^^^ Narayanaswami and Mcllwain 

 (1954), period of incubation 30 min. ^^^ Brody and Bain (1951), period of 

 incubation 30 min. ^^^ Clowes and Keltch (1951), period of incubation 

 40 min. ^'^ Christie et aL (1953), period of incubation 30 min. <®) 

 Vignais and Vignais (1957), period of incubation 40 min. ^^^Dianzani 

 and Scuro (1956), period of incubation 10 min. <io) Aldridge (1957), 

 period of incubation 30 min. 



require(d purely for the study of biochemical reactions. If require(i 

 to provi(ie information relating to physiological and biochemical 

 problems in the whole animal or organ, the obtaining of prepara- 

 tions which are undamaged and operate at 37 °C becomes important. 

 In this respect the preparative techniques of Aldridge (1957) and 

 the study of factors affecting the stabilization of mitochondrial 

 phosphorylation at 37 °C (Christie et aL, 1953; Gallagher et aL, 

 1956) are major contributions. 



The substrates supporting oxidative phosphorylation in mito- 

 chondrial preparations (Table 13) include all the members of the 

 tricarboxylic cycle. However, it should be noted that Christie et aL 

 (1953) and Aldridge (1957) found citrate unable to support 



