Immunity and Cutaneous Sensitization 39 



When a virulent strain <>l T. gypseum is inoculated cutaneously intp 

 guinea-pigs, the incubation period is lour to six days, and the period of 

 spread or development lasts seven to 10 days. A climax is reached 1>\ the 

 twelfth to sixteenth day. The lesion then begins to disappear spontaneously, 

 and healing is complete in 30 to 35 days. Considerable variations in the 

 time factors occur when different species of fungi are used and even with 

 different strains of the same species. The susceptibility, time for develop- 

 ment of a reaction it the animal is susceptible and the appearance of the 

 reaction are also variable when other laboratory animals such as kittens or 

 rabbits are employed. When guinea-pigs and rabbits are subjected to addi- 

 tional inoculations, the incubation period is shortened and the reaction 

 greater. Henrici found that intraperitoneal reinoeulation of live spores or of 

 trichophytin when the first infection had not quite healed resulted in a 

 diffuse generalized reaction of the skin. If interested in experimental prob- 

 lems in which laboratory animals are to be used, the reader is referred to 

 the original articles mentioned in the bibliography. 



BIBLIOGRAPHY 



Biberstein, H., and Epstein, S. : Ininiunreaktionen bei der menschlichen und tierexperimen- 

 tellen Oidiomykose der Haut, Arch. f. Dermat. u. Syph. 165:716, 1932. 



Bloch, B., in Jadassohn, J.: Handbuch der Haut- und Geschtectitskiankliciten (Berlin: Julius 

 Springer, 1928), vol. 11, pp. 300 and 564. 



Cormia, F. E., and Lewis, G. M.: Experimental aspects of penicillin sensitization, with spe- 

 cial reference to conjoint sensitization to superficial fungous disease, J. Invest. Dermat. 

 7:375, 1946. 



; Lewis, G. M., and Hopper, M. E.: Experimental aspects of penicillin sensitization: II. 



With reference to Schultz-Dale phenomenon, J. Invest. Dermat. 8:395, 1947. 



DeLamater, E. D.: Experimental studies with dermatophytes: III. Development and duration 

 of immunity and hypersensitivity in guinea-pigs, J. Invest. Dermat. 4:143, 1941; IV. 

 Influence of age upon allergic response in experimental ringworm in guinea-pig, ibid. 5:423, 

 1942. 



, and Benham, R. W.: Experimental studies with dermatophytes: I. Primary disease in 



laboratory animals, J. Invest. Dermat. 1:451, 1938; II. Immunity and hypersensitivity in 

 laboratory animals, ibid. 1:465, 1938. 



Fishman, H. C: Discussion of Katzenstein. 



Goodman, H., and Marks, I.: Reaction to trichophytin compared with reactions to othei 

 bacterial products, Arch. Dermat. & Syph. 31:819, 1935. 



Henrici, A. T.: Experimental Trichophytid, in Proceedings of the Third International Con- 

 gress for Microbiology (Baltimore: Waverly Press, 1940), p. 567. 



Jadassohn, W., and Peck, S. M.: Epidermophytide der Hande, Arch. f. Dermat. u. Syph. 

 158:16, 1929. 



; Schaaf, F., and Wohler, G.: Analyses of composite antigens by Schultz-Dale technic: 



Further experimental analyses of trichophytons, J. Immunol. 32:203, 1937. 



Katzenstein, L.: Specificity of skin tests in deep fungous infections, J. Invest. Dermat. 

 9:249, 1947. 



k\n her, W.: Ueber den diagnostischen Wert der intrakutanen Trichophy tinreaktion, Deutsche 

 med. Wchnschr. 62:138, 1936. 



Lewis, G. M., and Hopper, M. E.: Ringworm of scalp: IV. (a) Comparative reactions to 

 cutaneous tests with trichophytin in children with and without ringworm of the scalp; (b) 

 Evaluation of therapy with stock vaccines in types of infection resistant to treatment, Arch. 



