the Direct Examination 237 



or lour times. Examine il under the microscope. II the preparation is nol 

 clear, reheat it and examine it again. Repeat tliis until the tissue is clear 

 enough to provide satisfactory examination. The material is placed on one 

 end ol the slide so that the fingers are not burned when the slide is being 

 heated, and the potassium hydroxide is added cautiousl) to avoid flooding. 



As an alternative method il an immediate examination ol the mount is 

 unnecessary, the prepared slide ma\ be left warming over a microscope 

 lamp. 'This prevents disorganization ol the tissue to be examined. Care 

 should be taken thai evaporation docs not cause crystallization. II the slide 

 becomes dry, water may be added, rather than more hydroxide, so that the 

 crystals are redissolved. 



Many other methods and reagents have been described. Reports of some 

 are mentioned in the bibliography. 



BIBLIOGRAPHY 



\i mi w k /., J., and Gohny, W.: Uebcr eine vereinfachte Farbungsmethode zur Darstellung von 

 Fadenpilzen in Schuppen und Haaren in der ambulanten Praxis, Dermat. Wchnschr. 101: 

 1031, 1935. 



\\iw\. |.: Conservierungsfliissigkeiten und Einschlussmedien fiir Moose, Cloro und Cyano- 

 phyceen, Ztschr. f. wissensch. Mikr. 13:18, 1896. 



CoRNBLEET, T. : Reagent for demonstrating fungi in skin scrapings and hair, J. A. M. A. 95: 

 L743, L930. 



Kesteven, H. L.: New method of staining skin and hairs for detection of fungi, Brit. |. Dermat. 

 49:500, 1937. 



Langeron, \I.: Precis de microscopie (Paris: Masson & Cie, 1925). 



Linder, D. H.: An ideal mounting medium for mycologists, Science 70:430, 1929. 



Moore, M.: Mycologic technic in dcrmatologic practice, Arch. Dermat. & Syph. 34:880, 1936. 



Schubert, M.: Zur Farbung der Hautpilze, Dermat. Wchnschr. 105:1025, 1937. 



Swartz, J. II., and Conant, N. F. : Direct microscopic examination of skin: Method for de- 

 termination of presence of fungi, Arch. Dermat. & Syph. 33:291, 1936. 



