254 An Introduction to Medical Mycologij 



To tin's basic Formula may be added: 



Methylene blue 0.0002 Gm. 



Dextrose 1.0 Gm. 



The methylene blue is an indicator; the dextrose is for enrichment. 



This medium may be obtained from Baltimore Biological Laboratory, 

 432 North Calvert Street, Baltimore. 



BIBLIOGRAPHY 



Benham, R. W. : Cultural characteristics of Pityrosporum ovale: Lipophylic fungus, J. Invest. 



Dermat. 2:187, 1939. 

 Brewer, J. H.: Clear liquid mediums for "aerobic" cultivation of anaerobes, J. A. M. A. 



115:598, 1940. 

 Hodges, R. S.: Cultures of ringworm fungi on Sabouraud's proof mediums and on mediums 



prepared with American peptones and sugars: Comparative study, Arch. Dermat. & Syph. 



18:852, 1928. 

 Lewis, G. M., and Hopper, M. E.: Pigment production by fungi, Arch. Dermat. & Syph. 



44:453, 1941. 

 Sabouraud, R.: Les Teignes (Paris: Masson & Cie, 1910). 

 Southworth, W. H. : Specific chemical medium for pathogenic fungi, Arch. Dermat. & Syph. 



36:302, 1937. 

 Weidman, F. D., and McMillan, T. M.: Comparison of ingredients of ringworm culture 



mediums, Arch. Dermat. & Syph. 4:451, 1921. 

 ■ , and Spring, D.: Comparison of ringworm culture ingredients: II and III, Arch. Dermat. 



& Syph. 18:829, 1928. 

 Williams, J. W.: Effect of variation of ratios of dextrose to peptone on colonies of certain 



pathogenic fungi, Arch. Dermat. & Syph. 32:893, 1935; ibid. 34:15, 1936. 



2. INOCULATION OF MEDIUM 



The area of skin from which material is to be taken is washed with 70 

 per cent alcohol. Then it is scraped with the blade of a sterile scalpel, and 

 the material is transferred directly to the agar slant. The material is left 

 on the surface when the scalpel is cut several times across the medium. 

 We use this method for the culture of macerated skin, scales, nail tissue, 

 material from a moist, exuding surface and hair from the scalp. Hair can 

 probably be best removed from the beard by epilating forceps. When hair 

 is removed for the direct examination, a forceps is usually employed so 

 that the entire hair can be examined. The scalpel scraped over the tongue 

 gives one a good specimen from the mouth. Transferring the material im- 

 mediately from the patient to the agar gives a high percentage of cultures 

 free of contamination. 



A platinum loop of medium strength is useful in transferring a specimen 

 of stool to the surface of the agar slant. The same implement is useful in 

 transferring specimens of serum, spinal fluid, bile or sputum from specimen 

 bottles. Here it may again be emphasized that fresh material is desirable, 

 since most normal and pathologic body fluids are good mediums for the 



