CHAPTER XXXI 



Other Specific Skin Tests 



1. THE OIDIOMYCIN TEST 



THE precautions described for the use of trichophvtin are similar when 

 oidiomycin is used. The commercial vaccine (Lederle) should be 

 diluted in the ratio of 1:150 with sterile saline solution; 0.1 ee. of the ex- 

 tract is the test dose. In interpreting the quantitative reaction of the skin to 

 oidiomycin we read the test as for trichophvtin as follows: a cipher indicates 

 a negative reaction; a plus-minus sign an area of slight erythema approxi- 

 mately 0.5 cm. in diameter; 1 plus, 0.5 to 1 cm.; 2 plus, 1 to 1.5 cm.; 3 plus, 

 1.5 to 2 cm., and 4 plus, an elevated area of reaction 2 cm. or more in di- 

 ameter. The strongest reactions are those which remain for a week or more. 

 We have occasionally observed an immediate wheal reaction to oidiomycin. 



2. THE COCCIDIOIDIN TEST 



The vaccine is prepared from the growth on Bureau of Animal Industry 

 Svnthetic Media after two months at 37 C. A dilution is made of 1:1,000 

 normal saline to which is added merthiolate. The material should be passed 

 through a Berkefeld filter and tested for sterility. 



3. THE BLASTOMYCIN TEST 



The antigen is made by heat-killing the yeastlike growth obtained by 

 culturing B. dermatitidis at 37 C. A dilution of 1:1,000 is made with sterile 

 saline. The test dose is 0.1 cc. 



4. THE SPOROTRICHIN TEST 



The vaccine is made in a manner similar to trichophvtin. According to 

 du Toit, the colon v should be killed by heat and diluted with saline solution. 



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