CHAPTER XXVII 



Technic of Passive Transfer Test 



WALZER introduced the passive transfer technic to clinical practice. 

 The test is not extensively used in connection with fungous diseases 

 hut may be useful when direct testing with trichophytin and other extracts 

 is not feasible. 



1. TECHNIC 



1. Ten cc. of blood is withdrawn from the patient, using a dry syringe. 

 This blood is transferred to a sterile centrifuge tube. 



2. If plasma is desired, glass beads in the tube are shaken and the blood 

 is centrifuged immediately. 



3. If serum is desired, the clot is allowed to retract, the material being 

 kept in the ice box. When the clot has retracted the specimen is centrifuged. 



4. The serum or plasma is then removed aseptically and stored in the 

 ice box until used. It may be mixed with equal parts of normal saline. 

 Phenol (0.4 per cent) may or may not be added. 



5. The patient must be examined and found free from contagious or 

 infectious disease. In particular, a blood test for syphilis should be per- 

 formed before the serum or plasma is used on another subject. 



6. The recipient should be healthy, should not be subject to a major 

 allergy and preferably should not be harboring pathogenic fungi. 



7. The serum or plasma is injected intracutaneously into as many sites 

 as there are test substances. If the left side of the back is used for the 

 actual test, the right side in a symmetrical location can be utilized for the 

 control. The sites of injection are then ringed with mercurochrome or some 

 other dye. 



8. In 24 to 48 hours, the recipient is ready for the actual testing. The 

 material is injected intracutaneously, suitably diluted, into the exact sites 

 where the serum or plasma was previously introduced. If possible, not 

 only use the same needle puncture but carry the substance into the skin 



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