FINE STRUCTURE 45 



Extensive studies on pigment granules in coeruleus were made 

 by Weisz (1949a, 1950a). He found them to have a basophiUc core 

 of protein pigment, surrounded by a phosphoHpid shell, and to 

 give a negative test for RNA but positive for cytochrome oxidase. 



A B 



Fig. 9. Granular stripes adapting to space available. 



A. Specimen from which most of ectoplasm was removed, 

 granular stripes of the patch stretching to cover the endoplasm. 



The animal then regenerated. 



B . Photograph showing granular striping of nonuniform width 



and contour according to the space provided. 



The bright red appearance of these green particles in reflected light 

 he attributed to phase interference by the outer shell. Andrews 

 (1946) regarded the pigment granules of coeruleus as not mito- 

 chondrial; but Weisz concluded that they, as well as those of 

 Blepharisma, are mitochondria, basing this on their enzymatic 

 content, lipo-protein composition, apparent involvement in 

 metabolism, and especially their selectivity for Janus green B stain 

 (with no other bodies so staining). Admittedly it was difficult to 

 make distinctions in staining a green body green. 



A better test is the demonstration of villiform interior structure 

 typical of the protozoan mitochondrion. The electronmicrographs 

 of Faure-Fremiet et al. (1956) and Randall and Jackson (1958) 

 clearly reveal mitochondria in stentors, but these bodies appear too 



