RECONSTITUTION IN DISARRANGED STENTORS 223 



length appears. Even if the head had previously been excised and 

 the animals minced just before the anlage was due to appear, the 

 primordium v^as still not formed until considerable reorientation 

 had occurred. But if a stentor is minced and the mouthparts 

 excised at the same time the primordium can and often does appear 

 within the normal time of 4 hours, so that cutting of the striping 

 and its subsequent rearrangement does not seem to interfere in 

 any way with the activation and preparation of the cell for pri- 

 mordium formation. All the fine-stripe patches may not aggregate 

 in one place and therefore two primordia may be formed producing 

 a double stentor from a single. Oral regeneration seems to proceed 

 normally whenever an l.s.c. is estabhshed to determine where the 

 anlage is to be placed and it was noted that without exception the 

 primordium does appear in an l.s.c. Such loci may be much dis- 

 torted due to the original disarrangement, and the anlage faithfully 

 follows their contorted contour (Fig. 63B). The specimen therefore 

 does not wait until it has reestablished perfect order in the striping 

 but regenerates as soon as possible and makes further adjustments 

 later. 



When all but the primordium of regenerating stentors was 

 thoroughly minced there was no resorption of the anlage, which 

 continued to develop, though often slowly; but the membranellar 

 band formed was usually distorted. This indicates that the state 

 of activation is not nullified by severe cutting, but that orderly 

 striping is required for normal deployment of the developing 

 feeding organelles. Even if the regeneration primordium itself 

 was cut in two, the parts usually rejoined and development 

 continued to rather successful regeneration. 



Specific inhibition of oral primordium formation by intact 

 feeding organelles occurred even though the cell was minced. This 

 was demonstrated by grafting intact heads to singles and 2-masses 



no strong orienting influence on adjacent patches. Specimen 

 survived 8 days without reorganizing, and hence oral inhibition 

 of primordium formation was effective though lateral striping cut 

 into patches. 

 D. Mince graft of 5 whole stentors achieves axis by 6 hours, 

 regenerates as a doublet and begins dividing as a doublet 2 days 

 later. Integration of shape is better than in grafts of 5 not 



minced. 



