358 THE BIOLOGY OF STENTOR 



corner of the stentor to near its base is made and the two halves, 

 still attached at the posterior pole, are spread out widely (Fig. 99A). 

 Thus exposed, the nuclear nodes stand out more clearly than ever 

 and are rapidly teased out with the needle or sliced off with mini- 

 mum cytoplasm. All this can be done while leaving the oral struc- 

 tures entirely intact. When all visible nodes have been removed, the 

 two halves of the specimen are then brought together in normal 

 location to aid their rejoining in normal shape. Then the posterior 

 end of the cell is split apart and the last nodes searched for among 



Fig. 99. Enucleation and renucleation. 



A. a: Incision to enucleate coeruleus without disturbing 

 feeding orgenelles and with minimum loss of cytoplasm, h: 

 After cell is laid open, margins with macronuclear nodes are 

 excised or nodes teased out. c: As specimen heals together, 

 posterior end is opened to cut out remaining nodes obscured by 



carbohydrate reserves. 



B. In renucleation with nodes from same or a different species, 

 enucleated host is split open when endoplasmic sac with nodes 

 is available {a); the sac is broken against host wound, the endo- 

 plasms fusing; and nuclear nodes are then securely inside (c). 



