BIOLOGY OF PNEUMOCOCCUS 



mal density, virulence, and polysaccharide content. Pneu- 

 mococcal protein. Somatic carbohydrate or C Fraction. 

 Capsular polysaccharide: preparation of the specific poly- 

 saccharide of Type I Pneumococcus ; preparation of the 

 specific polysaccharide of Type II Pneumococcus; prepa- 

 ration of the specific polysaccharide of Type III Pneu- 

 mococcus. 634 



V. PREPARATION OF BACTERIAL ENZYMES CAPABLE OF DECOM- 

 POSING CAPSULAR POLYSACCHARIDES. 640 



vi. serological reactions. Agglutination. Precipitation: 

 optimal proportions ; nitrogen determination ; modified 

 routine; combining equivalents. Complement fixation. 

 Bactericidal tests. 641 



VII. POTENCY TESTS ON ANTIPNEUMOCOCCIC SERUM. United 



States Hygienic Laboratory, now National Institute of 

 Health : culture ; serum ; the test." League of Nations : ani- 

 mals; culture; serum. American Drug Manufacturers' As- 

 sociation: unit of serum; test dose of culture; the test; 

 interpretation of test; records and reports. Massachu- 

 setts Antitoxin and Vaccine Laboratory : culture ; serums ; 

 the test; interpretation of the test; protocols and records. 648 



VIII. STERILITY TESTS ON ANTIPNEUMOCOCCIC SERUM. Bulk 



sterility tests. Sterility tests on final containers. 662 



IX. PREPARATION OF DIAGNOSTIC ANTIPNEUMOCOCCIC RABBIT 



serum. Massachusetts Antitoxin and Vaccine Laboratory 

 method: vaccine; injection of rabbits; bleeding of rab- 

 bits ; testing of serum ; dilution of serum. 663 



BIBLIOGRAPHY 



665 



INDEX 771 



