Krumwiede and Mouse Taping 



Water bath- heat to coef- 

 ulate and c .tr.ct soluble 

 substance. 



[Sputum extract auered 

 over tu.pin( »rum. 



Krumwiada TtajpineJ 

 Tim. 15-20 min. 



Krumwiede 



Sabin 



Small drops of exudate 

 imtirtd on slid* with utmi 

 and fu,p'ng sere. 



Stain and examir 

 microscope. 



1 



A. 



afglu+inati 

 homologous tup*. 



Failure of agglutination 

 to develop is sufgeetive «n 

 group Ig-but not dafinite. 



Sabin Ti^pinf 



Time _ 5 hrs. 



[Sputum J 



I Emulsify with I 



I Saline in mortar | 



[ Inject Into live mouse | 



JL 



After 3-5 hrs. abdomen 

 punctured -exudate ob~ 



1 



After 5-24 hrs.- avenging 

 6~ 6 hrs.— mouse dias or is 

 Killed and autopsiad. 



Centrifuge washings and 

 pipatta off supernatant 



for precipitin test. 



Precipitin 



Suspend sediment in saline- 

 place in tubes containing 

 ecpjsl amounts of taping sera. 



T 



1 n m b . 



1 Lager supernatant over 

 tu>nf sera. 



III 



I Shake and incubate lh7 

 — 



ftimH 



Positiv* reaction (•filutinetion) 

 in tube indicates tup* of 

 orej.ni.m. (II in this c.e). 

 Failure of afflutinetion to 

 occur means pncumococcus 

 (roup rz, 



Positive reaction at juncture 

 of the two fluids indicates 

 tiipc. ( HI in this use). 

 Failure of reaction to ocerr 

 means (roup TS . 



Tub* •fflu+inatfon Tijpinf 



Time 5-24 hrs. 



averaging 6 _ 6hrs. 



Precipitin Tujpinf 



Tima — tame ae 

 tMaa agglutination. 



Courtesy of Massachusetts Department of Public Health 



CHART I. KRUMWIEDE AND SABIN METHODS OF TYPE 

 DETERMINATION 



