Neufeld Tliping 



Sputum 



Transfer fleck of sputum fo slide- 

 mix with fqping sera (rabbit) 

 and alkaline methujene blue. 



T 



III 



o o o 



Cover drops with cover 

 slips and examine under 

 oil immersion lens with 

 light partiallu, dimmed. 



Positive Reaction showing 

 swollen capsules in prepara- 

 tion with serum of homol- 

 ogous tups. 



Negative Reaction (capsules not 

 swollen) 



The absence of swollen capsules 

 in all 3 preparations suggests 

 Group nST pneumococci. 



Time 5-30 Minutes 



Courtesy of Massachusetts Department of Public Health 

 CHART 2. THE NEUFELD OR QUELLUNG METHOD OF TYPE DETERMINATION 



