Antipneumococcic Serum Potency Test - Protection Method - Type 



3r L.A B fc E-C. W. 



Sate 



l>-g-3t, 



Date test begun »V-S-3b Date teat finished lv-|v-3fc 



House weights vary from lb to VO grams 



Volume of test dilutions of serums Injected O'STcc. 



Test dilution of culture It 11,000 Volume Injected O-Scc 



Number organisms in test dose io.ooo Bomber lethal doses in test doae 80,000 



Serum Control Ho. 

 Serum Dilutions 



Pll. 



1 : iv. Sao 



Serum Lah. Ho. CPU B 

 Serum Dilutions 

 1: vr.ooo >'■ SO. OOP 



Dead at 



1 I h-rs. 

 £ 



S 

 S 



s 



s 

 s 



■S 



n 



%' 



s 

 S 



s 

 ^_ 



3 



1 



P&eumococcus Culture Controls 

 Broth used 1 1 - I 8 - 3 •» Seed culture usedPw H c J*liv-1-9Urolume seed used |Occ. 

 Vol. troth inoculated )5.occ No. hours grown 4'/v Turbidity jbweL- 



Virulence Test 

 0.5 cc. of following dilutions Injected! 



| •. 1 1 O -7vujlflc«rp- » : ilO -v«\xji«rY\_ I '. 1 I O O 



Ho. orgs, inject.: g I *.| 



Re.souT.I>eAl> At. v+Kts. «1 v4- 



+3 



Plate Counts 



I 



Plate 1 1 1 



Plate 2 5 



Serum dilutions fromtolSS'to II 

 Injections started \: i£? 



0.5 cc. of following dilutions elated: 

 1 1 o -ynjJuiio'^. \ *. 3 3 o -wiJuL-LyTv- | : 



It- 



Interpretation of Test 

 CP T T B C<rrCCe-^o cJUrvJC 



V I 



O O 



Culture dilutions from I '. 4-0 to l-:io 

 Injections finished V : ST O 



8oo 



•aa-jr 



jl*^-3o / W'cc. 



SAMPLE PROTOCOL OF A TYPE II TEST, MASSACHUSETTS 

 DEPARTMENT OF PUBLIC HEALTH 



