Antlpneumococcic Seram Potency Test - Protection Method - Type X 

 L ■ A . B (o E ■ C ■ W Date I V- T--3 b 



Date test began l> - v - 3 U Date test finished 1 7- - b - 3 b 



Mouse weights vary from lb to vO grams 



Volume of test dilutions of serums injected O-S'ce. 



Test dilution of culture I :vroo Volume injected OS" cc 



Bomber organisms in test dose4o»,oocMuniber lethal doses in test dose 100,000 



Serum Control So. P- II 

 Serum Dilutions 

 I '• I OQQ I : -uooo I : 4-onn 



Serum Lab. No. CP11B 

 Serum Dilutions . 



I : to, 000 I; vo.ooo i : fro, 000 



Dead at 4/3 Kts. b1 >fr 



bl b1 4-3 



S bl 4-3 



S 1v 48 



«S S bl 



S JS b1 



3 S b1 



S 5 bi 



S s fcT 



Pneumococcus Culture Controls 

 Broth used II -I 8-3b Seed culture used Pn IjMivi-atfolume seed used ( '• occ 

 Vol. broth inoculated is - , occ. . No. hours grown f'/t- Turbidity fa tmL- 

 Virulence Test 

 0.5 cc. of following dilutions injected: 



I ;>SO ->iu^lonrv_ I '. TSO-miW*«rv_ | IvSOO-mllLa 



Ho. orgs, inject. : .4" • * I 



Re-suur LtftB At 43hvs. 5 £ 



43 S S 



bl S S 



Plate Counts 



0.5 cc. offollowing dilutions plated;, ,_ . „* 



I : vSO -WJcOIS^ 1 : t Jo-r»vJlB->»^- » : vSoo-mJuL»v- 



Plate 1 3 I • 



Plate 2 £" t O 



Serum dilutions from Hfrq. to |>- •. frfr Culture dilutions froml.SVo v:vv 



Injections started v: 3 g Injections finished 3: | g 



Interpretation of Test 



SAMPLE PROTOCOL OF A TYPE I TEST, MASSACHUSETTS 

 DEPARTMENT OF PUBLIC HEALTH 



