INDEX 



797 



Toxin (s) ^cont.): 



lung-toxic principle, 98 



nature, 99 



necrotizing substance, 92, 98 



Pneumotoxin, 338 



properties, 95 



relation to protein poisons, 100-101 



relation to proteolysis, 94, 95 



relation to virulence of Pneumococ- 

 cus, 93 



stability, 97 



tolerance to, 95 



type-specificity, 100 



unsolved problems, 613-614 

 Transformation of Pneumococcus 



by animal inoculation, 155-160 



by heat-killed cultures, 157-158 



by vaccines, 158-160 



effect of sterile animal organs, 156 



in vivo, 158 



transformative principle, 160 



See also Dissociation 

 Transmutation of species, 169-177 

 Tribasic sodium phosphate in lysis of 



Pneumococcus, 59 

 Tropins. See Opsonins; Phagocytosis 

 Tubercle bacilli, polysaccharide in, 



262 

 Type determination 



agglutination, 123 



Amoss' method, 129-130 



Avery's method, 123 



Blake's method, 122 



Krumwiede method, 124-125, 630, 

 631 



mouse methods, 120-122, 628-630, 

 631 



multiple strains, 121 



Oliver's method, 126 



pooled serums, 129 



precipitin in blood, 125, 365-379 



precipitinogen in 

 sputum, 124-126 

 urine, 123-124, 366, 633-635 



protection tests, 120-122, 626-630 



Quellung phenomenon, 128, 131-132, 

 630, 632-633 



rabbit in, 121 



Type determination (cont.): 

 Sabin's method, 127-129, 628-631 

 slide agglutination, 127-131 

 urine precipitation, 123-124, 633- 



635 

 See also Serological classification 



Ulcus serpens, Pneumococcus in, 226 

 Ultracentrifugation 



in determining nature of antibodies, 

 414-417 



in purification of antipneumococcic 

 serum, 555 



in purification of cellular carbohy- 

 drates, 275 

 Ultrafiltration 



in determining molecular size of 

 antibodies, 417-418 



in purification 



of antipneumococcic serum, 555 

 of cellular carbohydrates, 275 

 Unit of immune serum, 28, 568, 586- 



587 

 Unit of polysaccharide-splitting en- 

 zymes, 311 

 United States Hygienic Laboratory, 



potency test, 567, 648-650 

 Unsolved problems, 613-622 



active immunity, production, 617- 

 618 



antibodies and animal species, 619- 

 620 



antipneumococcic serum 



methods of concentration, 620-621 

 potency tests, 621 



chemical constituents of Pneumo- 

 coccus, 615-616 



chemotherapy, 618 



dissociation, 614-616 



immunological response, 618-619 



pneumococcal antibody, nature, 620 



serological types, 614 



skin reactions, 619-620 



toxins, 613-614 



virulence, 616-617 

 Urine 



acetyl polysaccharide in, 284 



Pneumococcus in, 229 



