GENUS: ENDOLIMAX 241 



of E. nana. The amoeba cultivated by Gauducheau (1907, 1908), and named 

 by him (1907) E. phaf/ocytoides, was probably E. nana in the fresh stool, 

 but a free-living ama?ba in the culture. A further paper published by 

 him (1922) tends to confirm this opinion. As his description undoubtedly 

 applied chiefly to the cultivated form, it seems inadmissible to employ 

 his specific name phagocytoides for the human parasite, as Brumpt (1922) 

 and others have done. Thomson and Robertson (1925) have maintained 

 a strain of E. nana in Boeck and Drbohlav's L.E.A. medium for nineteen 

 days, during which fifteen subcultures were made. 



Kessel (1923rt, 1924f/) states that he has succeeded in infecting rats 

 and monkeys with E. nana. Chiang (1925) was unable to confirm these 

 observations on rats. 



ENDOLIMAX OF ANIMALS. 



Minchin (1910a) described as Malpighiella refrinyens a parasite he 

 had encountered in the Malpighian tubes of rat fleas {Ceratophyllus 

 fasciatus). It had an amoeboid phase, and also produced cysts which 



Fig. 112. — Malpighiella refringens from the Malpighian Tubes of the Rat Flea, 

 Ceratophyllus fasciatus : Am(EB0id and Three Encysted Forms ( x ca. 3,000). 

 (After Minchin, 1910, from Doflein, 1916.) 



resemble both in size and appearance those of E. nana (Fig. 112), 

 The cyst wall, however, is much thicker than that of E. nana. Noller 

 (1914) observed the organism in the Malpighian tubes of about 90 per cent, 

 of the dog fleas {Ctenocephahis canis) in Germany. From the fact that 

 I. 16 



