FALLACIES RESULTING FROM CONTAMINATION 273 



reported upon by Brumpt (19136). In the films it occurred as blue-staining 

 cytoplasmic bodies, sometimes pigmented and with one or two red-staining 

 masses. In two cases short flagella were noted. Furthermore, encapsuied 

 forms described as cysts were found. Brumpt noted that the films 

 contained the structures described by Franchini, as well as many bacteria, 

 some of which were actually within the cytoplasm of the flagellates. 

 The organism was placed in a new genus by Franchini as Hcemocystozoon 

 braziliense. The presence of encysted forms and the occurrence of 

 bacteria within the flagellates, as well as in other parts of the films, are quite 

 inconsistent with the assumption that the flagellate originated from the 



Fig. 122. — Trypanopsis maligmis, as seen in Dried Smears of the Liver stained 

 BY Eomanowsky Stain ( x ca. 4,000). (After Leger, M., 1920.) 



One form appears to be ingested by a leucocyte, but the association is probably accidental. 

 Similar forms were found in blood-films. 



blood. The use of a distilled water infected with flagellates and bacteria 

 could easily give rise to the appearances described, and this seems the 

 probable explanation of their origin. Franchini attempted culture from 

 the blood in N.N.N, medium, and no growth was obtained, a further 

 confirmation of the view expressed here of their extraneous origin. 



In the same category probably must be placed the flagellates described 

 by Leger, M. (1920), from a fatal case of pyrexia in a human being in French 

 Guiana. As figured by Leger, the organism appears as elongate flagellates 

 with single flagellum, rounded forms with one to three flagella, and rounded 

 forms without flagella (Fig. 122). Two chromatin masses were present, 

 and the flagellum arose from one of them. In some of the rounded, non- 



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