LEISHMANIA IN ANIMALS 441 



No infection of the blood or other organs could be detected. Two mice 

 which were fed on cloacal contents were said to have become infected. 

 Free leishmania forms are described as occurring in the heart blood and 

 bone marrow, and both these and leptomonas forms in the liver and spleen. 

 By employing N5ller's blood-agar plate method, cultures of the flagellate 

 were obtained from the cloacal contents of a chameleon. The flagellate 

 of the chameleon is of interest when compared with L. henrici, which 

 occurred, not only in the intestine of its host, but also in the blood. 

 Further investigation of the flagellate of the chameleon will probably 

 show that it also may occur in the blood-stream. 



Fig. 195. — Various Types of Leishmania chamceleonis found in Cloaca of 

 Chamwleon vulgaris (x 2,200). (After Wenyon, 1921; from Parasitology, vol xii.). 



Leishmania hemidactyli (Mackie, Gupta, and Swaminath, 1923). — 

 This parasite appears to be very similar to L. tarentolce. It was discovered 

 by the authors, who named it, in cultures made from the blood of the 

 Indian gecko, Hemidactylus gleadovii. Direct examination of the blood 

 failed to reveal any flagellates, and no mention is made of a concurrent 

 intestinal infection. A trypanosome named Trypanosoma hemidactyli 

 was also present in the blood. 



Franchini (1921a) records the presence of leptomonas and leishmania 

 forms of a flagellate in the rectum and cloaca, and also other parts of the 

 gut, of Lacerta ocellata. They were also said to be present in the leish- 

 mania form in the heart blood and liver. 



