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1. lODOACETATE AND lODOACETAMIDE 



The very marked accumulation of fructose-l,6-diP (see accompanying tab- 

 ulation) indicates the ability of the EM pathway to proceed through the 

 earher stages at this high concentration of iodoacetate. A similar picture 

 has been found in Chlorella exposed to 0.1 mM iodoacetate aerobically in 

 the dark (see tabulation below) (Kandler et at., 1961). Accumulation of 



triose-P's also occurs, as shown in Fig. 1-7 where the rapid changes in var- 

 ious intermediates and ATP may be observed. It was concluded that 0.1 

 mM iodoacetate produces here a fairly specific block at the 3-PGDH level. 

 The formation of P^^-labeled compounds in Helodea densa during 2-min in- 

 cubation in the dark following 9-min exposure to 0.4 mM iodoacetate was 



Fig. 1-7. Effects of 0.3 mM iodoacetate on various intermediates and 



nucleotides in Chlorella pyrenoidosa, after 1 hr incubation at pH 5.5 



in a medium containing glucose. (From Kandler et al., 1961.) 



