ANAEROBIC GLYCOLYSIS AND FERMENTATIONS 



75 



ATP (— 6.54) is approximately balanced by the increased phosphates 

 (+6.76). 



Several other more recent quantitative studies will be briefly presented 

 in order to illustrate the variations between different organisms and tissues. 

 During fermentation of glucose by Streptomyces olivaceus the concentrations 

 of the intermediates change about as expected when iodoacetate is present 

 (see accompanying tabulation) (Maitra and Roy, 1959). However, the rel- 



ative accumulations certainly do not conform to thermodynamic predic- 

 tions and the deviations may have resulted from phosphatase activity, since 

 the experiments were run over 96 hr. Even 7.5 mM iodoacetate appears to 

 block 3-PGDH fairly selectively over 20 min from the pattern of change 

 in the intermediates under aerobic conditions in yeast, where a greater ATP 

 supply probably facilitates phosphorylation of glucose (Holzer et al., 1955 a). 



