210 



1. lODOACETATE AXD lODOACET AMIDE 



Table 1-34 

 Inhibition of Enzymes Involved in Acetylcholine Metabolism 



Enzyme 



Source 



lodoacetate 



0/ 



/o 



[mM) Inhibition 



Reference 



this assay, choline and acetyl-CoA were used as substrates. In the total 

 reaction as it occurs in junctions, ATP is required for the formation of 

 acetyl-CoA, so that a second site, the generation of ATP, could also be 

 involved. The results on the cholinesterases in the table show quite con- 

 clusively that iodoacetate could not very well disturb junctional transmis- 

 sion by inhibition of these enzymes at the concentrations generally used 

 for blocking the EM pathway or occurring m vivo. 



