194 



1. lODOACETATE AND lODOACETAMIDE 



K+ loss, as can be seen in the figure. It is possible to explain at least a 

 major part of these effects on the basis of an increased permeability to the 

 ions and not necessarily an inhibition of active transport, but there is no 

 way to distinguish these mechanisms here. 



Fig. 1-13. Effects of various inhibitors on the ions of 



frog skin. The soUd lines represent Na+ and the dotted 



lines represent K+. DNP = 2,4-dinitrophenol; lA = iodo- 



acetate; and CX =^ cyanide. (From Huf el al., 1957.) 



Uptake of Cobalt by the Tissues of the Mouse 



An interesting study of the effects of iodoacetate on the uptake and dis- 

 tribution of Co^*^ in various organs of the mouse has been made by Maynard 

 (1958). Although the results are not easy to interpret, nevertheless this 

 type of investigation may be quite useful eventually in increasing our un- 

 derstanding of the mechanisms by which substances are distributed in the 

 whole animal. It was first established that 0.1 mM iodoacetate inhibits the 

 uptake of Co^° in mouse kidney slices and this was followed by a thorough 

 study of the in vivo response. Iodoacetate was injected intraperitoneally 

 (0.5 millimole/kg) and a few minutes later Co^" was given by the same 

 route; the animals were sacrificed at 24 hr. The results are shown in Table 



