EFFECTS ON CELL GROWTH AND PROLIFERATION 419 



loxan seems unlikely to be an effective carcinostatic agent, due to its in- 

 stability, but there is little information on this point. Grobon (1957) has 

 reported that intramuscular injections twice a week of alloxan (20-40 mg) 

 suppress the metastatic spread of endodermic and ectodermic carcinomas, 

 but one must be sceptical of such results because of the difficulty of evaluat- 

 ing metastatic changes. Finally, it may be noted that alloxan, in contrast 

 to most other SH reagents, does not produce blebbing of sarcoma ascites 

 cells (Belkin and Hardy, 1961). Most instances of definite inhibition of 

 cell proliferation thus involve the use of fairly high concentrations of al- 

 loxan, and in no case has alloxan been shown directly to be an effective 

 antimitotic agent. 



