76 ART. 3. S. IKEDA : CONTRIBUTIONS 



but with the punctured point, as happened in Group IL, Egg 

 No. 9. The main parts of the embryonic body were, therefore, 

 formed entirely upon the upper hemisphere (Fig. 94). 



Egg No. 4. (Fig«. 95 ar-g, Fig. 96). 



April 28, 1:35 p.m. : — (Fig. a). Punctured on both sides below the 

 level of the dorsal lip as in the eggs of the same number in 

 other groups. Punctures just seen in profile in the posterior 

 view ; about 20° below the level of the dorsal lip which was 

 8° or 9° below the equator. Plight puncture heavier and 

 exudation outside the envelope. Left puncture much lighter, 

 slightly protruded. 

 4:38 p.m. : — (Pig. h). Dorsal lip grown downward and increased 

 in length : its left limb longer and nearly reaching the left 

 ex-ovate which has grown a little larger ; its right limb still 

 some distance from the right ex-ovate. 



April 29, 8:45 a.m. :— (Fig. c=left side view, Fig. c£=ventral view). 

 Blastopore circle completed and ovate in shape, touching with 

 the acuminate end the right ex-ovate. Left ex-ovate grown 

 a little larger but firmly attached to the punctured point is 

 far outside the blastopore circle (about 70° from the left lip), 

 and curiously near the area of the segmentation cavity. 

 5:42 p.m. : — (Fig. e). Area of the segmentation cavity nearly 

 disappeared. Left ex-ovate in the same relative position. 

 Plight ex-ovate still attached to the right lip of the further 

 reduced blastopore. 



April 30, 9:07 a.m. : — (Fig. /). Blastopore become still smaller and 

 circular in shape, with the right ex-ovate yet closely attached 

 to the right lip. Left ex-ovate more remote from the left lip. 

 In Iront of the blastopore neural plate recognizable. 

 5:14 p.m. : — (Fig. g). Left ex-ovate approximately in the neck 

 region of the future embryo. Plight ex-ovate still attached to 

 the right lip of the now greatly reduced blastopore. The egg 

 unfortunately died after this. 



As the blastopore closed at the right point of puncture where 



the injury was greater, the growth of the blastopore rim must 



