TO THE EMBRYOLOGY OF AMPHIBIA. 75 



forward from the dorsal lip. At 7 a.m., fourth day (May 1), 

 blastopore closed just behind the tubercle. Embryonic body 

 not recognizable. 



Although the definite location of the embryonic body was not 



made out, it is evident that the blastopore was closed at the place 



of the greatest obstacle to the growth of the blastopore lips. 



Egg No. 3. (Figs. 93 a-h, Fig. 94). 



April 28, 12:50 p.m. : — (Fig. a). Punctured about midway from the 

 upper pole to the dorsal blastopore lip on the blastopore 

 meridian. Not only liquid contents but also some solid yolk- 

 substance exuded. 

 4:34 p.m. : — (Fig. b). Dorsal lip grown downward and increased 

 in length ; its right limb and ex-ovate nearer each other, as 

 has been often noticed before in similar cases. 



April 29, 8:30 a.m.: — (Fig. c=left side view, Fig. o? — posterior view). 

 Blastopore circle completed and grown small ; shape ovate, 

 acuminate toward the ex-ovate, which is found in the same 

 relative position to the dorsal lip. 

 5:38 p.m. :— (Fig. e). Blastopore greatly reduced in size. Area 

 of the segmentation cavity completely disappeared. Ex-ovate 

 in the same relative position. 



April 30, 9:04 a.m. : — (Fig. /). Blastopore nearly closed, leaving a 

 small oval yolk-plug exposed. Ex-ovate about 30° above it 

 in the same relative position. Medullary groove and folds 

 not distinct. 

 5:12 p.m. :— (Fig. g). Blastopore completely closed. General out- 

 line of the embryonic body well made out : at its tail end 

 is attached the ex-ovate. 



May 1, 7:04 a.m. : — (Fig. h). Embryo formation more advanced. Ex- 

 ovate in the same position as before. 



The blastopore was probably closed in this egg at about the 



point where the dorsal lip first appeared, if we may judge from the 



position of the ex-ovate. But curiously enough, the tail end of the 



embryo did not coincide with the closing point of the blastopore, 



