TO THE EMBRYOLOGY OF AMPHIBIA. 71 



pressure of the left hand needle during the operation which 

 was done with the right. After this, the course of develop- 

 ment was checked, and the egg was set aside. 



Egg No. 7. 



April 23, 8:20 + a.m. : — Punctured at the yolk-pole (as in Group I., Eggs 

 Nus. 1 and 9, and Group IV., Egg No. 1). The point of puncture 

 was however not at the actual pole, but slightly to the left when 

 seen in the posterior view. Exudation somewhat forcible. 



April 24, 7:22 a.m. : — Egg entirely recovered. Blastopore area and 

 area of the segmentation cavity diminishing in the normal 

 way. Ex-ovate near the middle point of the blastoporic area. 

 2:50 p.m. :— Blastopore now reduced to a small spot. General 

 outline of the neural plate faintly recognizable. Ex-ovate 

 close to the left blastopore lip. 



April 25, 5:20 p.m. : — Blastopore completely closed. General outline 

 of the embryonic body well established, entirely normal. Ex- 

 ovate attached to the left margin of the closed blastopore. 



Except that the axis of the embryo did not coincide with the 



blastopore meridian, but was bent slightly to the left, because the 



punctured point which became the tail end was slightly to the 



left, the results agree entirely with those in the above mentioned 



eggs in Groups I., and IV. 



Egg No. 8. 



April 23, 9:00 a.m. : — Punctured near the right limb of the dorsal 

 blastopore lip. Injury not severe, and a small quantity of the 

 egg-contents exuded outside the egg-envelope. 

 2:50 p.m. : — Dorsal blastopore lip increased in length and grown 

 a little downward, so that the right limb nearly reached the 

 ex-ovate. 



April 24, 7:30 a.m. : — Moderately reduced blastoporic area peculiar in 

 shape : ovate with the pointed end turned toward the ex-ovate 

 with which it was directly connected. Lateral and ventral lips 

 not distinct. 



