66 AKT. 3. S. IKEDA : CONTRIBUTIONS 



fortunately become detached and was found a short distance 

 forward from the tail end of the embryo. Embryo quite 

 normal. 



Although the detaching of the ex-ovate obscured the 

 final stage, there can be no^reasouable doubt that the closing 

 point of the blastopore was at, or near, the punctured point, 

 and so the location of the embryonic body nearly the same as 

 Egg No. 5 of this group or Egg No. 10 of Group IV. 



Second Group. (Figs. 87-92). 



The eggs of this group were obtained from a mass deposited 

 late on the morning of April 21. The dorsal blastopore lip ap- 

 peared at 8:10 a.m., April 23. Puneturings were carried out 

 between 8:20 and 9:30 of the same morning. 



Egg No. 1. (Figs. 87 u~d, Fig. 88). 



April 23, 8:20 a.m. : — (Fig. «). Punctured at the approximate middle 

 point of the yolk-pole (Cf. Group I., Eggs Nos. 1 and 9, and 

 Group IV., Egg No. 1). Needle-point somewhat blunt and 

 did not penetrate well. Only a small ex-ovate. 



April 24, 7:20 a.m. :— (Fig. b). Blastopore much reduced in size, and 

 somewhat oval in shape. Ex-ovate near the dorsal lip showing 

 that this lip has grown faster than the ventral. Another 

 unexpected ex-ovate discovered near the ventral lip, accounting 

 for the slow growth of that lip, and the shape of the blastopore. 

 3:30 p.m.:— (Fig. c). Blastopore much smaller. Dorsal ex-ovate 

 now excluded out of it and found to the left of the median 

 line of the neural plate consisting of a larger anterior, and 

 a smaller posterior globule arranged close together longitudinally. 

 Former no doubt the original ex-ovate, and the smaller formed 

 at the time of exclusion. Ventral ex-ovate at the ventral 

 blastopore lip. 



April 25, 5:40 p.m. :— (Fig. d). General outline of the embryonic body 

 distinct. Embryo, however, somewhat incomplete and its axis 



