TO THE EMBRYOLOGY OF AMPHIBIA. 65 



hemisphere as in Egg No. 1 of this and Group IV. Exuda- 

 tion rather less than usual. 



Twenty-four hours later, ex-ovate enormously elongated, 

 within the vitelline membrane, into a long streak the base of 

 which was attached to the left margin of the moderately closed 

 blastopore. The point in the vitelline membrane through 

 which puncturing was effected shifted considerably upward 

 from the dorsal lip. Careful examination showed that as 

 after puncture I had placed the egg with the yolk hemisphere 

 to one side, the egg rotated within the vitelline membrane to 

 its natural position, and in so doing the ex-ovate was gradually 

 elongated into a long streak by additional exudation. I tried 

 to restore the egg to the proper position, but the exudation 

 outside the outer envelope had become firmly attached to 

 cotton-fibres in a bed of which substance the egg had been 

 placed. So the egg was left as it was. 



Another twenty-fuur hours, some more exudation which 

 seemed, however, completely detached from the egg-surface. 

 Embryo seems normal in its formation beneath the large, but 

 detached ex-ovate. 



The final closing point of the blastopore and the location 

 of the embryonic body were somewhat obscured by the exuda- 

 tions, but as the ex-ovate was seen distinctly attached to the 

 dorsal lip up to the evening of the second day after puncture, 

 we may suppose that the final point of the blastopore closure 

 was, as usual, at the punctured point (in this case, the yolk- 

 pole), and that the embryo must have been formed as in 

 other cases of this kind. 



Egg No. 10. 



Punctured on the ventral median line about GS°- below 

 the equator, i.e. somewhat lower than in Group IV., Egg No. 

 10 (Figs. 75 «-/'). 



Twenty-four hours later, blastopore diminished to a very 

 small circular spot to the ventral margin of which the ex-ovate 

 was attached. 



After another twenty-four hours, the ex-ovate had un- 



