64 ART. ?. S. TKEDA : CONTRIBUTIONS 



Egg No. 6. 



April 17, 2:30 + p.m.: — Punctured in the middle line, close below the 

 dorsal blastopore lip (Cf. Group IV., Egg No. 6, Figs. 72 a-g). 

 Blastopore finally closed at the point of puncture, and 

 the embryo stretching forward from this point was distinctly 

 shorter than normal, though not as short as in Group IV., 

 Egg No. 6. 



or» 



The results are therefore exactly like those in the egg of 

 the corresponding number in Group IV. 



Egg No. 7. 



Not punctured, as there were 1 large, and 3 or 4 small 

 natural ex-ovates in the anti-blastoporic region. Twenty four 

 hours later, they had, however, all become detached from the 

 egg and attached to the inner surface of the vitelline mem- 

 brane. Hence quite useless for the present investigation. 



Egg No. 8. 



Punctured closely above the first dorsal blastopore lip as 

 in Group IV., Egg No. 7 (Figs. 73 a-f, Fig. 82). In this 

 egg the injury was slightly to the right of the middle line 

 and lighter. Twenty-four hours later, blastopore circular and 

 already small ; ex-ovate attached to its right margin. Hence 

 stage like Fig. 73 c missed, if present. Another twenty-four 

 hours, ex-ovate attached to the right margin of the closed 

 blastopore. Embryo short but otherwise normal. 



The results are therefore absolutely the same as those in 



Group IV., Egg No. 7. 



Egg No. 9. 



Chiefly instructive in regard to the formation of ex-ovates. 

 Punctured at the approximate lowest point of the yolk 



