EREMASCACEAE 173 



sterile horse serum. Lange (1921) used egg medium with 2% glucose and 

 1% glycerol, reporting colonies small, brownish yellow, dry, center elevated, 

 margin flattened, edge like a rampart, confluent near water of condensation. 

 No fermentation of sugars, only glucose and sucrose utilized. Ammonia 

 produced from peptone. Milk coagulated, gelatin rapidly liquefied. 

 Zymonema Molardi (Salvat & Fontoynont) Dodge, n. comb. 

 Endomyces Molardi Salvat & Fontoynont, Bull. Soc. Path. Exot. 15: 311- 

 320, 1922. 



Endomyces Molardi Fontoynont (nomen nudum) Bull. Mem. Soc. Chirurg. 

 Paris 48: 439-442, 1922. 



Isolated from lesion on leg of man inoculated by scratching, (?) at first 

 a fistula, then an ulcer, with depigmentation. Lesion finally cured by ex- 

 ternal applications of methylene blue for about one month. Inoculation on 

 thigh of a guinea pig caused abscess which healed spontaneously. Intraperi- 

 toneal injection caused a nephritis which was fatal in 34-44 days. Organism 

 recovered from the blood. 



Hyphae occasionally 2-3 fields long, 2.5-3.5/i. in diameter, irregularly septate 

 or fragmented, appearing- nearly empty, tinted slightly pale rose by Gram's 

 method. Yeast forms visible. Hyphae 2-6fx in diameter, contracted as septa, 

 thick-walled, very rich in glycogen. Filament may terminate in a simple cell, 

 a group of short, thick-walled, nearly round cells, one to two times as thick 

 as the penultimate cell; or it may end by a spherical cell, 8-22/t in diameter, 

 thick-walled, staining with aqueous eosin (chlamydospore). These chlamydo- 

 spores appear occasionally in groups or short chains. Ascospores appear on 

 glycerol agar or in liquid of glycerol-carrot. Asci spherical or ellipsoid, 4- 

 spored, rarely 8-spored. 



On glycerol agar, growth rapid, colony thick, creamy, ivory white, ele- 

 vated with little deeper yellow craters in the middle and never reaching the 

 side of the tube. On Sabouraud maltose, growth as on glycerol but colony 

 dirty, yellowish white. On Sabouraud glucose, growth less abundant, with 

 slightly elevated polycyclic plateau, center creamy, white, glistening, then 

 opaque. On fifteenth day fine radiations from central cone. On carrot with 

 glycerol, from streak, growth appears like white porcelain, is thick, glistening, 

 creamy, composed of a mass of granular colonies. Hyphae on walls of tube 

 in fifth month, the whole surface is invaded and becomes crateriform, moist 

 below, dryer above. Growth on potato and turnip with glycerol same as for 

 carrot. Growth from gelatin stab appears like inverted fir tree. On surface 

 plateau 8 mm. in diameter, three zones: (1) outer, 1 mm. broad, fine radiat- 

 ing lines, (2) higher, 3 mm. wide, abrupt drop to outer zone, (3) uppermost, 

 3 mm. broad, granular. On horse serum, growth is meager, old ivory in color. 

 On solid media, only yeastlike cells appear. In liquids a slight pellicle and 

 very fragile ring appears, sediment is flocculent and abundant, liquid remains 

 clear. On lactose bouillon, growth is good ; even after 5 months lactose is not 

 exhausted. Medium gradually becomes acid. Growth in glycerol bouillon 



