176 MEDICAL MYCOLOGY 



On potato glycerol, medium, darkened; serum not liquefied, milk coagulated 

 in 24 hours. In Raulin's solution, sediment, but not turbidity. Drigalski- 

 Conradi slightly acid in 4 days. On neutral red agar, gas in 24 hours, color 

 change in 4 days. No indol. No fermentation of sugars. Acid produced from 

 glucose, fructose, galactose, lactose, and arabinose, no action on mannite. 

 sucrose, raffinose, and inulin. 



Zymonema bucalis (Niiio & Puglisi) Dodge, n. comb. 



Monilia hucalis Niiio & Puglisi, Semana Med. 34: 222-229, 1927. 



The lesions began as perleche, extending gradually over the buccal mucosa 

 which became covered by white plaques, slightly adherent, not continuous, 

 suggesting clots of curdled milk, extending into the tonsillar crypts, produc- 

 ing a burning sensation. 



Yeast cells and hyphae 2.5-3/x, little branched. In cultures yeast cells 

 3-7 X 2-5/x; asci spherical, 10/a in diameter, with 2-4 ascospores. Hyphae de- 

 veloping from thick-walled cells, flexuous, little branched, 1.5-3/a in diameter; 

 blastospores lateral; large terminal ehlamydospores present. 



Colonies at 37° C. on Sabouraud agar, potato-8% glycerol, carrot, and 

 potato agar, circular, confluent, moist, white, little elevated. On liquid media 

 (broth, potato decoction, 2% peptone solution and maltose-peptone solution) 

 turbidity, with the formation of white clots, which settle to the bottom. 

 Drigalski-Conradi medium becomes red, then decolorized; litmus sugar agars 

 become red, then decolorized, and finally become blue. Acid on glucose, 

 sucrose, and lactose, not on mannite. Sucrose not inverted, starch digested. 

 Sugars not fermented. Milk coagulated on the third day, no liquefaction of 

 gelatin nor of coagulated serum. 



Zymonema Cruzi (Froilano de Mello & Paes) Dodge, n. comb. 



Endomyces Cruzi Froilano de Mello & Paes, Arq. Hig. Pat. Exot. 6 : 51-60, 

 1918. 



Isolated from sputum of patient suffering from bronchitis and asthma. 



In sputum cells 4-8 x 2-4/^, rarely spherical, with refringent granules. No 

 hyphae or spores present. On potato, cells ovoid or spherical, pseudomycelia 

 50-80/A long', simple or branched. Spherical or reniform asci present, 2-4- 

 spored. On simple agar, spherical cells in chains of 8 to 16, no asci. 



On 1% agar + 6 drops of 10% NaOH, mycelial filaments definitely septate, 

 100/A long with terminal ehlamydospores or lateral conidia at the septa, and 

 arthrospore formation. On 10 gm. agar + 5 drops 10% NaOH, some yeast 

 cells, mycelium, terminal ehlamydospores. On 10 gm. agar + 4 drops 10% 

 NaOH, feeble development, yeast cells, no true mycelium, asci abundant. 



On simple agar, feeble development, small milk-white colonies. On mal- 

 tose agar (Sabouraud), colony white, then yellowish, verrucose. Colonies on 

 potato, circular, elevated, wax color, opaque. Broth becomes cloudy after 72 

 hours with flocci in suspension, forming a filiform sediment. On Raulin liquid, 

 thin whitish pellicle becoming thicker and chalky spotted, with small white 



