EREMASCACEAE 177 



points above and below with streamers 1-2 cm. long protruding into the liquid, 

 finally breaking off and forming filiform sediment at bottom of the tube. 

 Glucose, maltose, and sucrose fermented. 



Zymonema Alvarezsotoi (Mazza & Niiio) Dodge, n. comb. 



Monilia Alvarezsotoi Mazza & Niiio, apud ]\Iazza, Niiio, Quintana & Bem- 

 aseoni, Bol. Inst. Clin. Quiriirg. Univ. Buenos Aires 6: 180-214, 38 figs., 1931. 



Generalized mycosis of native of Argentina. Fungus isolated from caseous 

 lumps in feces, from lumps in the sputum and from the urine. Patient finally 

 succumbed. Pathogenic to white rat, guinea pig, in intraperitoneal injection, 

 to rabbit intravenously or applied to the mucous membranes. In one rabbit 

 subcutaneous injection resulted in subcutaneous nodules which were surgi- 

 cally removed. The animal recovered completely. 



On Sabouraud solid media, yeast cells mostly round, variable in size, some 

 sprouting. No hyphae or asci observed. On coagulated human serum, after 

 one month, many yeast cells, mostly round, 1-10/a in diameter. Rare ovoid 

 forms. Few hyphae, composed of chains of fusiform, granular cells of vari- 

 able size. Lateral branches at septa. Occasionally two large cells, approxi- 

 mately of equal size, and with thick membrane, are joined by a narrow neck 

 [isogamous copulation?]. Morphology in Gougerot gelatin slightly different. 

 Hyphae predominate. These are variable in size, flexuous, composed of chains 

 with lateral ramifications. Some spherical or ovoid cells are united directly 

 to the hyphae or by little pedicels [immature asci?]. There are intercalary 

 or terminal swellings resembling chlamydospores. Some cells are cylindric 

 with rounded ends, 10 x 2/i,, vacuolate. In some filaments, internal spores like 

 oidia. Optimum temperature for growth 30-37° C, no growth at 50° C. 

 Gram-positive or negative. With Leishman stain, protoplasm sky blue, 

 chromatin granules garnet. 



On Sabouraud glucose agar, colony rugose yellowish. Same on Sabouraud 

 maltose. Good growth on agar and carrot. On potato, growth also good with 

 darkening of medium. No growth on Drigalski medium. On potato, with 

 8% glycerol, good growth in 24 hours with darkening of medium. Colony 

 white, humid, covering the whole surface. Pellicle on top of liquid and deposit 

 at bottom of tube. On carrot, with 8% glycerol, abundant growth in 24 hours, 

 colonies white, humid, covering whole surface of medium. Some days later 

 on upper part of edge some white, agglutinated hyphae appear. Pellicle on 

 surface of liquid ascending walls of tube. On Gougerot gelatin stab, rugose, 

 caramel-colored pellicle on surface of medium, presently darkening on top. 

 On plain gelatin stab at 15° C, dendroid growth along whole length of stab. 

 On gelatin streak, good growth, best at the bottom where some lateral arbor- 

 escences appear. Along coagulated human serum stab, growth slow and dend- 

 roid. In plain broth and Sabouraud broth, turbidity and sediment in clots. 

 In acid Raulin's solution, sediment in clots without turbidity. Starch paste 

 unaltered. No fermentation of sucrose, rafiinose, lactose, maltose, mannite, 

 sorbite, dextrin, or inulin. Slight acidity but no fermentation with glucose, 



