ENDOMYCETALES 155 



cultures, hyphae septate, 1-5/x in diameter, multinucleate, sometimes showing 

 raquet mycelium (Fig. 26). Clilamydospores sing^ly or in chains, intercalary 

 or lateral, sessile or pedicellate, 3-10/i, in diameter, rarely terminal, then 3-10 

 X 6-20/i. Conidia lateral, either sessile or pedicellate, spherical to pyriform, 

 2-8/A in diameter. No trace of sexual org^ans present. Asci terminal, lateral 

 or intercalaiy, at first spherical or clavate 5-18/a in diameter, smooth and 

 thick-walled at first, becoming" pitted, then spinose and finally tuberculate on 

 aerial mycelium, while those beneath the substrate remain smooth and thick- 

 Availed (Fig. 26, 5-19). The tubercles are very variable, sometimes resembling 

 germ tubes, but so far as known they are functionless. Asci with an indefinite 

 number of spherical spores. 



On more acid agars, colonies white, cottony, growth poor, and submersed, 

 asci not observed. On malt extract agar, colony light isabelline, with radial 

 furrows, margin woolly. On Sabouraud agar, colony light isabelline, cottony, 

 suggesting pleomorphic colonies of dermatophytes. On serum agar, colony 

 white, moist, with deep radial fun^ows and flat margin. Sediment but no 

 pellicle with broth, no fermentation or acid production, litmus milk alkaline 

 without coagulation or digestion, no liquefaction of gelatin. 



Histoplasma pyriforme (Moore) Dodge n. comb. 



Posadasia pijrifonnis ]Moore, Ann. Missouri Bot. Gard. 21: 347, 348, 1934. 



Isolated from histoplasmosis in Iowa by Hansmann & Schenken (1933). 



In general morphology close to H. capsulafum but asci pyriform, smaller, 

 6-12 X 12-26/A, usually about 10 x 22/^ (Fig. 27). 



On more acid agars, growth cottony, white, heaped up in the center. On 

 malt extract agar, colony isabelline, cottony, flat zonate, center elevated to 

 cerebriform. On Sabouraud ag-ar, colony isabelline, zonate, cottony, with a 

 woolly margin. On serum agar, colony cottony, light isabelline, with a flat 

 moist margin. On peptone glucose broth both sediment and pellicle produced ; 

 on lactose broth, sediment but no pellicle. No fermentation or acid produc- 

 tion in sugars, litmus milk alkaline without coagulation or digestion, no lique- 

 faction of gelatin. 



PARACOCCIDIOIDES 



Paracoccidioides Almeida, Annaes Fac. Med. Sao Paulo 5: [1-19] 9 pis., 

 1930. 



Vegetative characters both in tissue and in culture similar to Coccidioides. 

 In the tissues of the host, after rapid nuclear division in the ascus, the nuclei 

 migrate to and through the ascus wall, pushing out small ellipsoid to spherical 

 structures which are abjointed as spores. This process, similar to that re- 

 ported in Actonia and suggestive of conditions in Protomyces, is quite reminis- 

 cent of the formation of partial sporangia in the higher Mucoraceae (see pp. 

 102, 103). Ascus formation has not yet been observed in vitro. 



This tendency for the formation of spores outside the ascus seems not 

 to have developed further in this family, although in the Protomycetaceae, 



