EREMASCACEAE 181 



Isolated from severe pleiiropiilmonary infection and bronchopneumonia with 

 abundant j^easts appearing in the sputum. Producing local pyogenesis in rab- 

 bit and guinea pig. 



Cells spherical, rarely ellipsoid, mostly 3-9 x 1.5-4.5/i. When actively bud- 

 ding, cells are 15-18 x 4-5/i., with the formation of pseudomycelium but no true 

 mycelium on turnip, liver decoction, and glycosuric urine. In other media, 

 especially carrot and potato, cells separate early. On turnip, filamentous 

 forms found, spherical cells about 6/* in diameter, asci abundant. Irregular 

 allantoid forms found, also durable cells. Elongate forms on glycerol arti- 

 choke as well as on turnip. Budding forms abundant in liquid media, espe- 

 cially in the pellicle of old cultures. No trace of copulation before the formation 

 of the ascospores, which are 4 per ascus and measure 2-2. 5/a. Asci 7-8 x 5ft. 

 The ascospores swell and begin budding, still without any trace of copulation. 

 No especial cultural conditions seem necessary for ascospore formation. 



On Sabouraud agar, colonies white, punctiform, rapidly confluent, forming 

 a white, creamy, shining, flat surface elevated more than 1 mm.; margins 

 crenulate. Colonies on potato grayish white, punctiform, spherical, very much 

 elevated above the surface. As each colony grows rapidly, it becomes acumi- 

 nate in appearance and is confluent with neighboring colonies. When the 

 medium dries, the colonies become chalky white. On carrot, development is 

 more rapid than on potato. Colonies white, rapidly confluent into a varnished, 

 creamj^ surface. On turnip, growth at first similar to that on potato, then 

 colony becomes prominent, surface mammillate, pebbled, remaining grayish 

 white as the medium dries out. On artichoke, growth is slow but otherwise 

 resembles that on carrot. Glycerol, on vegetable media, somewhat inhibits 

 growth. On gelatin, combined with Lasseur's medium, colonies grayish white, 

 develop slowly and are very slowly confluent, if at all. On liver decoction 

 gelatin, grayish white, elevated, punctiform colonies. Development is rapid 

 and abundant. On gelatin, combined with normal Raulin solution, colonies 

 grayish Avhite, rapidly confluent to give the appearance of shagreen. In pepto- 

 glycerol broth, development is slow, slight turbidity at first, with deposit of 

 flocci at the bottom. Lasseur's medium is not especially favorable, develop- 

 ment is as in pepto-glycerol broth. On Courmont's medium with glucose, 

 development is rapid, sediment abundant, pellicle thick; with galactose and 

 lactose, sediment even more abundant ; with sucrose, sediment less marked, 

 pellicle feeble ; with maltose, sediment very marked, pellicle conspicuous ; with 

 inulin growth, slow at first, with pellicle and sediment finall}^ appearing; with 

 starch deposit, development slow. Grown in Sartory's mutton liver decoc- 

 tion, organism causes grayish floccose sediment, leaving liquid clear. In 

 glycosuric urine, containing 10 gm. glucose per liter, abundant deposit ap- 

 peared, liquid clear with some gas evolution, then appearance of pellicle. Ring 

 also after glucose had fermented. In normal Raulin 's solution, a powdery 

 white deposit appeared on the walls and bottom of container. Fermentation 

 occurred with glucose, fructose, galactose, lactose, maltose, and sucrose. Slight 



