EREMASCACEAE 167 



Z3rmonema capsulatum (Dodge & Ayers) Dodge, n. comb. 



Endomyces capsulatus Dodge & Ayers in Rewbridge, Dodge & Ayers, Am. 

 Jour. Path. 5: 349-364, Pis. 71-73, 1929. 



Isolated from granular, flesh-colored nodule on the surface of the medulla 

 in a case diagnosed as meningoencephalitis witli a complicating pulmonary 

 tuberculosis. On postmortem examination the lower surface of the cerebel- 

 lum, pons, medulla, and cranial nerves, also an old scar on left thigh, were 

 found to be covered with these nodules of mycotic origin. Spinal fluid not 

 involved. Not fatal to rabbits and guinea pigs. Intraperitoneal injection 

 into mice fatal in 3-4 weeks. 



In lesions, mycelium exclusively budding cells. In new cultures and acid 

 media, mycelium consists of thin-walled liyphae, 3.5-4/x in diameter, cells 

 nearly isodiametric (Fig. 32, 3). Hyphae often somewhat contorted. On 

 neutral, high protein media, hyphae, branched, 1.4-1.5/a in diameter, cells long, 

 mostly 2-5 nucleate (Pig. 32, 11, 18). Sprout cells, 3.5-5/*, abundant on acid 

 media, absent on neutral. Chlamydospores, terminal on short branches or in 

 chains (Fig. 32, i), develop from raquet mycelium. Mycelial cells 2.6-3/i, in 

 diameter with diameter of 6.5-7/* at swollen end. On corn meal agar, large 

 spherical cells, 11.3/* in diameter develop on the ends of short branches; walls 

 thick but contents appear degenerating. Conidia sessile, pyriform, 5.5-6.5/t, 

 developing irregularly along mycelium, usually near septum. Most abundant 

 on neutral media with protein nitrogen. "Endospores" ellipsoid, 2.6-3.2/*, on 

 the hyphae in Sabouraud glucose agar, formed by rounding up protoplasts, no 

 spore wall observed, deeply staining. Copulation heterogamous (Fig. 32, 6). 

 A cell of a filament swells to 11/t in diameter, fuses with an apparently little 

 differentiated cell (4.8/*) on a hypha (3.6/*) nearby. Asci found in cultures on 

 Sabouraud glucose agar about nineteen weeks old. Asci terminal on long fila- 

 ments, 8-spored, subspheric, 7x9/* (Fig. 32, 4, 8, 17). Ascospores hyaline, 

 spherical, smooth, 2/* in diameter. 



On wort agar (bacto, pH 4.5), there was no growth. On wort agar (pH 

 slightly higher), surface of colony moist, shining, j^eastlike at first, becoming 

 dull or powdery in age. Growth slow, less than 1 cm. in 6 weeks, elcA^ated, 

 convolute to cerebriform, mycelium very irregularly branching, septate, cells 

 short. Not much change after 5 months. On malt extract agar (bacto, pH 

 about 4.7), growth slow, scarcely covering surface in 19 weeks, compact thin 

 felt on older portion, sparse or fine powderj^ surface on younger portion. 

 Mycelium more filamentous than in wort agar, very few yeast cells. On 

 Sabouraud dextrose agar (bacto pH 4.74) and Sabouraud 's conservation agar, 

 growth more rapid, colony 2 cm. in diameter in 2 weeks, white, well-developed 

 at inoculum, surrounded by loose, cottony zone with dense, cottony periphery. 

 Mycelium filamentous, hyaline, branched, composed of clavate cells, 2-4.5/* in 

 short diameter. Hyphal tips swollen. On corn meal agar, growth similar to 

 that on Sabouraud 's agar, less developed and slower, mycelium very slender, 



