262 MEDICAL MYCOLOGY 



On peptoglycerol broth, pellicle is formed with pseudomycelium and a 

 sediment of spherical or ovoid cells. No aseospores seen on gypsum block. 

 Sucrose inverted. Glucose and fructose fermented, but not maltose, lactose, 

 or galactose. Almost no growth and no action on coagulated serum. Gelatin 

 not liquefied. 



Castellania africana (Macfie) Dodge, n. comb. 



Monilia africana Macfie, Ann. Trop. Med. Parasitol. 15: 275-277, 1921. 



Isolated from feces in a case of obstinate diarrhea. Patient's tongue red, 

 irregularly fissured, indented by teeth and partly eroded. Throat red. Feces 

 canary yellow and frothy. Castellania abundant. Entamoeba coli and Blasto- 

 cystis enterocola also present. 



Cells ovoid and somewhat elongate, 4.5 x 1.6/^ [average of measurements 

 of 10 cells] . On solid media, yeastlike cells predominate with a few branched, 

 septate hyphae. In liquid media, hyphae most common. Gram-positive. Not 

 acid-fast. 



On glucose agar, after 24 hours, white, very fluid growth with dull, pel- 

 licle-like surface. Growth slower and less abundant under anaerobic condi- 

 tions. On potato, growth is dull and white with a whitish efflorescence appear- 

 ing later. In broth and peptone water, sediment whitish, medium clear. Surface 

 pellicle in broth only. Glucose, fructose, and dextrin fermented. Gelatin not 

 liquefied. 



Castellania platensis (Peruchena) Dodge, n. comb. 



Monilia platensis Peruchena, Semana Med. 36: [1-31] 10 jigs., 1929. 



Isolated from a patient who suffered from cough, expectoration, loss of 

 strength and weight. Lung involved. Pathogenic for rabbits. 



Yeast cells predominate at first, finally hyphae ; septate, elongate cells, 

 15-20 X 2-3/x up to 50 x 4-5/i,. No asci observed. Cells rounded or ovoid, 4-6/x 

 in diameter, increase by sprouting. Gram-positive. 



On simple agar at 38°, small, rounded, isolated colonies, 2-4 mm., slightly 

 elevated in the center, finely granulose at the periphery, dull white, becoming 

 confluent. On litmus lactose agar, abundant development with a slight nile 

 green color in the substratum. On glucose neutral red agar, good develop- 

 ment, colonies take dye, no color change. On Sabouraud glucose agar, colo- 

 nies dull white, 1-2 mm. Endo medium red with colonies taking dye. On 

 gelatin stab, 18 small colonies, 1-2 mm. in diameter. No pigmentation, colonies 

 superficial. Broth shows no turbidity or odor, milky white sediment formed, 

 also sometimes there is a weak pellicle from floating islets. In bile broth, no 

 turbidity, but there is a precipitate. No indol formation. Ferments fructose 

 and maltose. Acid formation strong with galactose and arabinose, slight with 

 fructose, maltose, and mannose. Glucose not fermented. On coagulated 

 human sernm, there is slight development, no liquefaction or pigmentation. 

 Milk slightly acidified without coagulation. Gelatin not liquefied. 



Castellania alba (Castellani & Chalmers) Dodge, n. comb. 



Monilia alha Castellani & Chalmers, Man. Trop. Med. ed. 3, 1089, 1919. 



