268 MEDICAL MYCOLOGY 



Parasaccharomyces intestinalis (Mattlet) Dodge, n. comb. 



Blast ode ndrion mtestinale Mattlet, Ann. Soc. Beige Med. Trop. 6: 16-17, 

 1926. 



One of the organisms isolated from stools of patients suffering from 

 dysentery in Belgian Congo. 



In potato decoction at 37° after 3 days, yeast cells ovoid, 2.5-3.5 x 5-8/i, 

 vacuolate, sprouting to give spherical cells, 2-3/t in diameter which soon 

 elongate ; hyphae, simple or slightly branched, composed of 12-20 cells with 

 small area of contact. After 30 days the yeast cells contain fat droplets and 

 some are thick-walled, spherical, 7-8/a in diameter with a crown of granula- 

 tions as in Atelosaccharomyces pyogenes. The hj^phae become firmer, not easily 

 dissociated, with larger areas of contact between cells. 



On Sabouraud agar at 37° C, colony round, white, dull. Later the center 

 yellows and crumples, with radial folds at the lobate margin and verrucose 

 projections into the medium. On gelatin stab, colony develops similarly with 

 abundant granulations, as large as 1 mm. in diameter, along stab. In potato 

 decoction, white lumpy sediment. Optimum for culture, 37° C. Milk slightly 

 acidified. Fermentation of glucose, fructose, maltose, galactose ; none of 

 sucrose, lactose, mannite, dextrin, inulin. Gelatin liquefied with evolution of gas. 



Parasaccharomyces irritans (Mattlet) Dodge, n. comb. 



Blast odendrion irritans A Mattlet, Ann. Soc. Beige Med. Trop. 6: 18-19, 

 1926. 



Isolated from the sputum in several cases of mycosis of the respiratory 

 tract with dry cough in long drawn-out fits of varying degrees of severity. 

 Treatment with potassium iodide, emetine, and arsenicals gave improvement, 

 although the parasite generally remained in the sputum. 



In sputum, yeast cells, spherical or ovoid, 2-5/a in diameter, sprouting or 

 in groups. After 3 da3\s at 37° in potato decoction, single cells, ovoid or 

 spherical, with numerous granulations and rather rare types of cellular group- 

 ings. Spherical cells up to 6/* in diameter, ovoid cells 3.5 x 7/a. Groupings 

 consist of an axis of elongate cells, 10 x Sfi, from whose septa grow either 

 lateral filaments, or spherical cells, or chains of cells. Between the elongate 

 cells sometimes grow intercalary spherical cells. Free ends of cells always 

 rounded. Same after 30 days. Optimum temperature for growth 37° C. 



On Sabouraud agar, colony round, cream colored at the smooth margin 

 with yellow center, surface smooth and slightly shining, the center wrinkling 

 slightly in age, no submerged mycelium. On gelatin stab, small cup of lique- 

 faction after 20 days. In depths numerous granulations along the stab, medium 

 displaced. In potato decoction, sediment of grayish white clots which break 

 up on agitation. Fermentation of glucose, fructose, maltose, galactose, sucrose, 

 none of lactose, mannite, dextrin, inulin. Slight acid formation with milk but 

 no coagulation. Slight gas with gelatin, also liquefaction. 



The following strain B described by Mattlet differs in minor particulars 

 but scarcely seems deserving of varietal rank. 



