EREMASCACEAE IMPERFECTAE 269 



Blast odendrioii irritans B Mattlet, Ann. Soc. Beige Med. Trop. 6: 18-20, 

 1926. 



Monilia sp. (case V) Mattlet, Ann. Soc. Beige Med. Trop. 4: 173, 1925. 



Isolated from cases clinically similar to those of the species. In the case 

 V Soter, Aspergillus giganteus was isolated by Mattlet at the same time as 

 Blast odendrioii irritaris. 



After 3 days at 37° C. in potato water, many spherical cells, 6/x in diameter, 

 and ovoid cells 7-8 x 4/t, with a large central vacuole. Rare elongate cells, 

 8 X 3/A, forming hyphae when attached end to end. At the septa, sprout single 

 elongate or spherical cells. After 30 days all cells show fatty granulations. 



On Sabouraud agar, appearance as in P. irritans A, except margin lobate 

 and tufts of hyphae penetrating deeply into substrate. Action on sugars 

 differs from P. irritans A only in intensity. Litmus milk at first slightly acid, 

 then alkaline. Gelatin liquefied, gas evolved. 



Parasaccharomyces Talicei Dodge, n. sp. 



Monilia sp. Talice & Mackinnon, Bol. Inst. Clin. Quiriirg. Univ. Buenos 

 Aires 4: 502-519, 13 figs., 1928. 



Isolated from the sputum of a case simulating pulmonary tuberculosis 

 without showing presence of Mycobacterium. Pathogenic to rabbits on in- 

 travenous injection, to white rat on intraperitoneal injection, but not found 

 pathogenic to guinea pig. 



Rare hyphae in sputum. Blastospores present. In culture, blastospores 

 of variable size, spherical, 2-11/^ in diameter, averaging 5-6/t, ovoid 5-7/a in 

 diameter, sometimes forming chains in cultures more than 3 days old. Blasto- 

 spores germinate by septate hyphae, 10-30 x 2/t, which in turn give rise 

 terminally and laterally to hyphae and blastospores. Hyphae increase in 

 number as the culture ages, but yeast forms always predominate. In very 

 old cultures the hyphae form arthrospores. Protoplasm generally granular 

 and shows oil droplets. Gram-positive, stained with May-Giemsa stain. 

 Optimum temperature for growth 36°-38° C, grows anywhere between 18° 

 and 40° C., does not grow above 45° C. 



Giant colony, after 16 days, is 4-5 cm. in diameter, approximately circular, 

 granular at the center, with radiating furrows which end in indentations at 

 the margin, generally white but assumes color of the medium. On plain or 

 Gorodkova agar, a moist and white covering. On potato, colonies of irregular 

 outline, verrucose. On caiTot, surface colonies which grow rapidly and be- 

 come folded. On Sabouraud glucose agar, growth very good, forming a moist 

 thick covering which acquires the color of the medium. On ordinary Sabour- 

 aud agar or Sabouraud with maltose, groAvth similar but less abundant. On 

 Sabouraud conservation agar, growth poor, verrucose. In glucose broth, a 

 very light pellicle which clings to sides of tube. Similar on Raulin's liquid. 

 Acidification and fermentation with glucose, maltose, galactose, sucrose, inulin. 



